MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NOP (OPRL1) RECEPTOR
1 vial (2 x 106) frozen cells
Expression vector containing full-length human OPRL1 cDNA (GenBank Accession Number NM_000913.3) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin, 800 μg/mL G418
Stable after minimum two months continuous growth
Background: NOP, ORL1 or OPRL1 (opioid receptor-like 1 receptor) is also known as nociceptin receptor, orphanin FQ receptor and KOR-3 (kappa 3-related opioid receptor). It is a receptor for the neuropeptide nociceptin/orphanin FQ and has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. In vivo experiments have demonstrated that nociceptin modulates a variety of biological functions including nociception, food intake, memory processes, cardiovascular and renal functions, spontaneous locomotor activity, gastrointestinal motility, anxiety and neurotransmitter release at peripheral and central sites.
Application: Functional assays
Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, monitored on Flexstation III. Figure 2. Dose-dependent inhibition of forskolin- stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti- FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Calo et al. (2000) Pharmacology of nociceptin and its receptor: a novel therapeutic target. Br J Pharmacol 129:1261-1283.
Ciccocioppo et al. (2003) The nociceptin/orphanin FQ/NOP receptor system as a target for treatment of alcohol abuse: a review of recent work in alcoholpreferring rats. Physiol Behav 79:121-128.