MULTISCREEN™ β-ARRESTIN STABLE CELL LINES

HUMAN RECOMBINANT GPR132 RECEPTOR

Product Information

Catalog Number:
CA1066

Lot Number:
CA1066-081619

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio 11891)

Host cell:
HEK293T β-Arrestin2

Transfection:
Expression vector containing full-length human GPR132 cDNA (GenBank Accession Number AF083955) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 250 µg/mL hygromycin

Stability:
Stable for a minimum of 1 month in continuous culture

Data Sheet

Background: GPR132 (also known as G2A) is a proton-sensing receptor that is broadly expressed in the appendix, lymph nodes and other tissues. It may also be a receptor for oxidized free fatty acids. It appears to play a role in regulating certain cells of the autoimmune system by responding to the extracellular acidification characteristic of tissues suffering from malignant cell growth or inflammation

Application: Functional assays

Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, measure with MultiscreenTM β-Arrestin Assay Kit (Multispan MSBAK01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti- FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Obinata, et al. (2005) Identification of 9-Hydroxyoctadecadienoic Acid and Other Oxidized Free Fatty Acids as Ligands of the G Protein-coupled Receptor G2A.JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 280, NO. 49, pp. 40676–40683,

Murakami et al. (2004) G2A Is a Proton-sensing G-protein-coupled Receptor Antagonized by Lysophosphatidylcholine. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 279, No. 41, Issue of October 8, pp. 42484–42491,

FOR RESEARCH USE ONLY.
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