MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPR120 RECEPTOR
1 vial (2 x 106) frozen cells
CellBanker 2 (Amsbio 11891)
Expression vector containing full-length human GPR120 cDNA (GenBank Accession Number: NM_181745.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: GPR120 is a G protein-coupled receptor for the long-chain free fatty acids. GPR120 mediated calcium mobilization, Erk1/Erk2 activation and GLP1 secretion. Unsaturated long-chain FFAs had a dose-dependent stimulatory effect, and α-linolenic acid was the most potent. GPR120 and GLP1 colocalized in human colonic intraepithelial neuroendocrine cells, and GPR120 may mediate dietary FFA- stimulated GLP1 secretion.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent internalization of surface receptor. Figure 3. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Fredriksson et al. (2003) Seven evolutionarily conserved human rhodopsin G protein- coupled receptors lacking close relatives. FEBS Lett 554:381-388.
Hirasawa et al. (2005) Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120. Nature Med 11:90-94.