MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT 5-HT4A RECEPTOR
1 vial (2 x 106) frozen cells
Amsbio Cellbanker 2
Expression vector containing full-length human HTR4 cDNA (GenBank accession number NM_001040169.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin
Stable in culture for minimum of 2 months
Background: The human serotonin receptor 5-HT4 is a G protein-coupled receptor. 5-HT4 receptors can be found in the central nervous system and in peripheral tissues such as the alimentary tract, urinary bladder, heart and adrenal gland. 5-HT4 ligands may prove to be therapeutic in the treatment of disorders such as cardiac arrhythmias, irritable bowel syndrome and urinary incontinence.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Talley (2003) Evaluation of drug treatment in irritable bowel syndrome. Br J Clin Pharmacol 56:362-369.
Van den Wyngaert et al. (1997) Cloning and expression of a human serotonin 5-HT4 receptor DNA. J Neurochem 69:1810-1819.