MULTISCREEN™ STABLE CELL LINES

RAT RECOMBINANT TA1 RECEPTOR

Product Information

Catalog Number:
C1357

Lot Number:
C1357-102610

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Full-length rat TAR1 cDNA (GenBank Accession Number AF380186).

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 100 µg/mL hygromycin, 100 µg/mL zeocin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: TAR1 (Trace Amine Receptor 1) or TA1 is a receptor for trace amines. The receptor can be activated by endogenous trace amines as well as metabolites of the biogenic amine neurotransmitters. Trace amines are biogenic amines present in very low levels in mammalian tissues. Although some trace amines have clearly defined roles as neurotransmitters in invertebrates, the extent to which they function as true neurotransmitters in vertebrates has remained speculative. Trace amines are likely to be involved in a variety of physiological functions that have yet to be fully understood. Trace amine receptors are potential direct targets for drugs of abuse, including amphetamine and 3,4-methylenedioxymethamphetamine (MDMA).

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation.

References:

Miller et al. (2005) Primate trace amine receptor 1 modulation by the dopamine transporter. J Pharmacol Exp Ther 313:983-994.

Borowsky et al. (2001) Trace amines: identification of a family of mammalian G protein- coupled receptors. Proc Natl Acad Sci USA 98:8966-8971.

FOR RESEARCH USE ONLY.
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