MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT MC1 RECEPTOR

Product Information

Catalog Number:
C1340

Lot Number:
C1340-09262012

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Full-length Human MC1R cDNA (GenBank Accession Number NM_002386.3) with FLAG-tag sequence at the N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: The melanocortin 1 receptor (MC1R), also known as melanocyte-stimulating hormone receptor (MSHR) or melanotropin receptor, is a seven transmembrane G-protein coupled receptors expressed on the surface of melanocytes MC1 receptors binds to a class of pituitary peptide hormones known as the melanocortins. Binding of melanocortins to five subtypes of melanocortin receptors exert a large array of central and peripheral effects. MC1R is one of the key proteins expressed in skin and hair and involved in regulating skin and hair color. Activation of MC1R by agonist stimulates the synthesis of eumelanin by a process melanogenesis, primarlily activating adenylate cyclase. MC1R has a potential role in anti-inflammatory and melanoma treatment. Further studies of the MC1 receptor are essential in gaining a better understanding of the mechanism and in vivo regulation of pigmentation and anti-inflammatory functions.

Application: Functional assays

Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Beaumont KA, et al (2010) Melanocortin MC1 receptor in human genetics and model systems. European Journal of Pharmacology 660:103-10

Neumann AG, et al. (2001) MC(1) receptors are constitutively expressed on leucocyte subpopulations with antigen presenting and cytotoxic functions. Clin Exp Immunol 126(3):441-6.

FOR RESEARCH USE ONLY.
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