MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MC1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human MC1R cDNA (GenBank Accession Number NM_002386.3) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: The melanocortin 1 receptor (MC1R), also known as melanocyte-stimulating hormone receptor (MSHR) or melanotropin receptor, is a seven transmembrane G-protein coupled receptors expressed on the surface of melanocytes MC1 receptors binds to a class of pituitary peptide hormones known as the melanocortins. Binding of melanocortins to five subtypes of melanocortin receptors exert a large array of central and peripheral effects. MC1R is one of the key proteins expressed in skin and hair and involved in regulating skin and hair color. Activation of MC1R by agonist stimulates the synthesis of eumelanin by a process melanogenesis, primarlily activating adenylate cyclase. MC1R has a potential role in anti-inflammatory and melanoma treatment. Further studies of the MC1 receptor are essential in gaining a better understanding of the mechanism and in vivo regulation of pigmentation and anti-inflammatory functions.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Neumann AG, et al. (2001) MC(1) receptors are constitutively expressed on leucocyte subpopulations with antigen presenting and cytotoxic functions. Clin Exp Immunol 126(3):441-6.