MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT D5 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human DRD5 cDNA (GenBank Accession Number NM_000798.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: The human dopamine receptor D5 is a D1-like receptor and its activation stimulates adenylyl cyclase activity. It has a 10-fold higher affinity for dopamine than the D1 receptor. The D5 receptor is expressed in neurons in the limbic regions of the brain, and has been implicated in ADHD and addiction disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Bergson et al. (1995) Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain. J Neurosci 12:7821-7836.
Dearry et al. (1990) Molecular cloning and expression of the gene for a human D1 dopamine receptor. Nature 347:72-76.
Laplante et al. (2004) Reduction in Acetylcholine Release in the Hippocampus of Dopamine D5 Receptor-Deficient Mice. Neuropsychopharmacology 29:1620-1627.