MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT D2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human DRD2 cDNA (GenBank Accession Number NM_000795.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum two months continuous growth.
Background: The human dopamine receptor DRD2 (D2) is a G protein-coupled receptor for dopamine. It can be found on postsynaptic dopaminergic neurons that are centrally involved in reward-mediating mesocorticolimbic pathways. Signaling through dopamine D2 receptors governs physiological functions related to locomotion, hormone production, and drug abuse.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Grandy et al. (1989) Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc Nat Acad Sci USA 86:9762-9766.
Neville et al. (2004) Identification and characterization of ANKK1: a novel kinase gene closely linked to DRD2 on chromosome band 11q23.1. Hum Mutat 23:540-545.
Usiello et al. (2000) Distinct functions of the two isoforms of dopamine D2 receptors. Nature 408:199-203.