MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT D1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human DRD1 cDNA (GenBank Accession Number NM_000794) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: The human dopamine receptor D1 (DRD1 or D1) is a G-protein- coupled receptor. It is expressed most abundantly in the brain areas such as caudate, nucleus accumbens and olfactory tubercle. The D1 receptor regulates neuronal growth and development, mediates some behavioral responses, and modulates dopamine receptor D2-mediated events.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Dearry et al. (1990) Molecular cloning and expression of the gene for a human D(1) dopamine receptor. Nature 347:72-76.
Huang et al. (20008) Significant association of DRD1 with nicotine dependence. Hum Genet 123:133-140.
Xu et al. (1994) Dopamine D1 receptor mutant mice are deficient in striatal expression of dynorphin and in dopamine-mediated behavioral responses. Cell 79:729-742.