MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT 5-HT6 RECEPTOR

Product Information

Catalog Number:
C1331-1

Lot Number:
C1331-031809

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human HTR6 cDNA (GenBank accession number NM_000871) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: Human 5-HT6 receptors are G protein-coupled receptors. The cDNA encodes a 440-amino-acid polypeptide. The 5-HT6 receptors are expressed exclusively in brain regions such as striatum, cortex, nucleus accumbens, olfactory tubercle, hippocampus, hypothalamus, amygdala and cerebellum. The 5-HT6 receptors appear to regulate GABAergic, glutamatergic and cholinergic neurotransmission and are involved in the regulation of cognition, feeding and sleep/wake regulation. They may also play a role in mediating the effects of some antipsychotic agents, and in modulating sensory gating which is often affected in schizophrenic patients.

Application: Functional assays

Figure Legend: Dose-dependent increase of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Wesołowska A (2002) In the search for selective ligands of 5-HT5, 5-HT6 and 5-HT7 serotonin receptors. Pol J Pharmacol 54:327-341.

Woolley ML, Marsden CA, and Fone KC (2004) 5-HT6 receptors. Curr Drug Targets CNS Neurol Disord 3:59-79.

FOR RESEARCH USE ONLY.
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