MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT 5-HT6 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human HTR6 cDNA (GenBank accession number NM_000871) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: Human 5-HT6 receptors are G protein-coupled receptors. The cDNA encodes a 440-amino-acid polypeptide. The 5-HT6 receptors are expressed exclusively in brain regions such as striatum, cortex, nucleus accumbens, olfactory tubercle, hippocampus, hypothalamus, amygdala and cerebellum. The 5-HT6 receptors appear to regulate GABAergic, glutamatergic and cholinergic neurotransmission and are involved in the regulation of cognition, feeding and sleep/wake regulation. They may also play a role in mediating the effects of some antipsychotic agents, and in modulating sensory gating which is often affected in schizophrenic patients.
Application: Functional assays
Figure Legend: Dose-dependent increase of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Wesołowska A (2002) In the search for selective ligands of 5-HT5, 5-HT6 and 5-HT7 serotonin receptors. Pol J Pharmacol 54:327-341.
Woolley ML, Marsden CA, and Fone KC (2004) 5-HT6 receptors. Curr Drug Targets CNS Neurol Disord 3:59-79.