MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT 5-HT4 RECEPTOR

Product Information

Catalog Number:
C1330

Lot Number:
C1330-012610

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human HTR4 cDNA (GenBank accession number NM_000870.4) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin

Stability:
Stable after minimum two months continuous growth

Data Sheet

Background: The human serotonin receptor 5-HT4 is a G protein-coupled receptor. 5-HT4 receptors can be found in the central nervous system and in peripheral tissues such as the alimentary tract, urinary bladder, heart and adrenal gland. 5-HT4 ligands may prove to be therapeutic in the treatment of disorders such as cardiac arrhythmias, irritable bowel syndrome and urinary incontinence.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Talley (2003) Evaluation of drug treatment in irritable bowel syndrome. Br J Clin Pharmacol 56:362-369.

Van den Wyngaert et al. (1997) Cloning and expression of a human serotonin 5-HT4 receptor DNA. J Neurochem 69:1810-1819.

FOR RESEARCH USE ONLY.
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