MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT 5-HT4 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human HTR4 cDNA (GenBank accession number NM_000870.4) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin
Stable after minimum two months continuous growth
Background: The human serotonin receptor 5-HT4 is a G protein-coupled receptor. 5-HT4 receptors can be found in the central nervous system and in peripheral tissues such as the alimentary tract, urinary bladder, heart and adrenal gland. 5-HT4 ligands may prove to be therapeutic in the treatment of disorders such as cardiac arrhythmias, irritable bowel syndrome and urinary incontinence.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Talley (2003) Evaluation of drug treatment in irritable bowel syndrome. Br J Clin Pharmacol 56:362-369.
Van den Wyngaert et al. (1997) Cloning and expression of a human serotonin 5-HT4 receptor DNA. J Neurochem 69:1810-1819.