MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT 5-HT1B RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human HTR1B cDNA (GenBank accession number NM_000863.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: The human serotonin receptor 5-HT1B is a G protein-coupled receptor. 5-HT1B receptors are present in many parts of the central nervous system, but most notably, can be found in the basal ganglia, striatum, and frontal cortex. 5- HT1B receptors inhibit the release of many neurotransmitters, such as serotonin, GABA, acetylcholine, and glutamate. 5-HT1B ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and aggression.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hamblin et al. (1992) Molecular cloning and functional characterization of a human 5- HT1B serotonin receptor: a homologue of the rat 5-HT1B receptor with 5-HT1D-like pharmacological specificity. Biochem Biophys Res Commun, 184:752-759.
Hamon et al. (1990) The main features of central 5-HT1 receptors. Neuropsychopharmacology 3:349-360.
Ruf et al. (2009) The 5-HT(1B) receptor: a novel target for the pathophysiology of depression. Curr Drug Targets 10):1118-1138.