MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT 5-HT1A RECEPTOR

Product Information

Catalog Number:
C1319

Lot Number:
C1319-042310

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human HTR1A cDNA (GenBank accession number NM_000524.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid
nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: The human serotonin receptor 5-HT1A is a G protein-coupled receptor. 5-HT1A receptors can be found mainly in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells. References:
Hamon et al. (1990) The main features of central 5-HT1 receptors. Neuropsychopharmacology 3:349-360. Savitz et al. (2009) 5-HT(1A) receptor function in major depressive disorder. Prog Neurobiol 88:17-31. Stam et al. (1992) Genomic organization, coding sequence and functional expression of human 5-HT2 and 5-HT1A receptor genes. Eur J Pharmacol 227:153-162.

FOR RESEARCH USE ONLY.
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