MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CYSLT2 RECEPTOR

Product Information

Catalog Number:
C1307

Lot Number:
C1307-082509

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Full-length human CYSLTR2 cDNA (GenBank Accession Number NM_020377)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 200 µg/mL hygromycin

Stability:
Stable after minimum two months continuous growth

Data Sheet

Background: Cysteinyl leukotriene receptor 2 (CysLTR2) is a receptor for cysteinyl leukotrienes. There are two receptors for cysteinyl leukotrienes, CysLTR1 and CysLTR2. CysLTRs have a clear role in pathophysiological conditions such as asthma, allergic rhinitis and other nasal allergies, and have been implicated in a number of inflammatory conditions in cardiovascular and gastrointestinal diseases.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Takasaki et al. (2000) The molecular characterization and tissue distribution of the human cysteinyl leukotriene CysLT(2) receptor. Biochem Biophys Res Commun 274:316-322.

Capra (2004) Molecular and functional aspects of human cysteinyl leukotriene receptors. Pharmacol Res 50:1-11.

FOR RESEARCH USE ONLY.
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