MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NK3 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human TACR3 cDNA (GenBank Accession Number NM_001059.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: Three membrane receptors that recognize these tachykinins have been described, termed TACR1, TACR2, and TACR3 (or NK3). Their endogenous ligands are substance P, neurokinin A, and neurokinin B, respectively. Interest in the tachykinins and their respective receptors has been stimulated by their potential role in bronchial asthma.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hopkins et al. (1991) Isolation and characterisation of the human lung NK-1 receptor cDNA. Biochem Biophys Res Commun 180:1110-1117.
Huang et al. (1992) cDNA sequence and heterologous expression of the human neurokinin-3 receptor. Biochem Biophys Res Commun 184:966-972.