MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT NK2 RECEPTOR

Product Information

Catalog Number:
C1304

Lot Number:
C1304-073005

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human TACR2 cDNA (GenBank Accession Number NM_001057) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: The tachykinin receptor 2 (TACR2 or NK2) is a receptor for the tachykinin family of peptide neurotransmitters. NK2 receptor selectively binds neurokinin A (substance K), which is predominating in human and animal airways. It has been shown to regulate circular muscle contraction. NK2 can be found in tracheal and gastric tissues.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Sasai and Nakanishi (1989) Molecular characterization of rat substance K receptor and its mRNAs. Biochem Biophys Res Commun 165:695-702.

Morten et al. (1991) Human NK-2 receptor gene maps to chromosome region 10q11- 21. Hum Genet 88:200-203.

FOR RESEARCH USE ONLY.
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