MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NK2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human TACR2 cDNA (GenBank Accession Number NM_001057) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: The tachykinin receptor 2 (TACR2 or NK2) is a receptor for the tachykinin family of peptide neurotransmitters. NK2 receptor selectively binds neurokinin A (substance K), which is predominating in human and animal airways. It has been shown to regulate circular muscle contraction. NK2 can be found in tracheal and gastric tissues.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Sasai and Nakanishi (1989) Molecular characterization of rat substance K receptor and its mRNAs. Biochem Biophys Res Commun 165:695-702.
Morten et al. (1991) Human NK-2 receptor gene maps to chromosome region 10q11- 21. Hum Genet 88:200-203.