MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT NK1 RECEPTOR

Product Information

Catalog Number:
C1303

Lot Number:
C1303-070111

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human TACR1 cDNA (GenBank Accession Number NM_001058.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable for minimum of two months in culture

Data Sheet

Background: NK1 (or tachykinin receptor TACR1) is a selective receptor for substance P with relatively higher affinity for neurokinin A compared to neurokinin B (100- and 500-fold lower). Neurons in lamina I of the spinal dorsal horn express the NK1 receptor for substance P and mediate hyperalgesia, an enhanced sensitivity to painful stimuli. Although substance P does not mediate the signaling of acute pain or hyperalgesia, it is essential for the full development of stress-induced analgesia and for an aggressive response to territorial challenge. It is also suggested that substance P is important for orchestrating the response of the animal to major stressors such as pain, injury or invasion of territory. Modulation of substance P activity offers a radical new approach to the management of depression, anxiety and stress. The substance P receptor is highly expressed in areas of the brain that are implicated in these behaviors.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Lecci et al. (2000) Peripheral actions of tachykinins. Neuropeptides 34:303-313.

Quartara and Maggi (1997) The tachykinin NK1 receptor. Part I: ligands and mechanisms of cellular activation. Neuropeptides 31:537-563.

Quartara and Maggi (1998) The tachykinin NK1 receptor. Part II: distribution and pathophysiological roles. Neuropeptides 32:1-49.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com