MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT PTH1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human PTH1R cDNA (GenBank Accession Number NM_000316.2) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: Parathyroid hormone receptor (PTH1R) functions as a receptor for parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP). It is expressed in high levels in bone and kidney and mediates mineral ion homeostasis as well as the paracrine actions of PTHrP, which is important in the process of endochondral bone formation. Defects in PTH1R can cause chondrodysplasia and enchondromatosis. The development of novel PTH receptor ligands could prove to be valuable in the treatment of diseases such as osteoporosis and hypercalcemia.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Mannstadt et al. (1999) Receptors for PTH and PTHrP: their biological importance and functional properties. Am. J. Physiol. 277 (5 Pt 2): F665-75.
Langub et al. (2001) Parathyroid hormone/parathyroid hormone-related peptide type 1 receptor in human bone. J. Bone. Miner. Res. 16: 448-456.