MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT RXFP2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human RXFP2 cDNA (GenBank accession number: NM_021634)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable for minimum of two months in continuous growth
Background: The relaxin family peptide receptor RXFP2, also known as LGR8, is a 754-amino acid 7 transmembrane protein. RT-PCR detected RXFP2 expression in various organs of the reproductive system along with the brain, kidney, thyroid, muscle, peripheral blood cell, and bone marrow. RXFP2 is known to be important in supporting germ cell function in the testis and ovaries. Additionally, RXFP2 mutations are linked to osteoporosis in men, suggesting that it plays a central role in bone physiology.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-RXFP2 antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hall et al. (2006) Relaxin family peptide receptors, RXFP1 and RXFP2, modulate cAMP signalling by distinct mechanisms.. Mol Pharmacol (1):214-26.
Kumagi et al. (2002) INSL3/Leydig insulin-like peptide activates the LGR8 receptor important in testis descent.. J Biol Chem 277: 31283-31286.