MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GNRH RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GNRHR cDNA (GenBank Accession Number NM_000406) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM + 10% FBS + 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: Gonadotropin-releasing hormone receptor (GnRHR) is a receptor for gonadotropin-releasing hormone (GnRH), also known as luteinizing hormone- releasing hormone (LHRH) and prolactin release-inhibiting factor (PIF). GnRHR is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Gametes and preimplantation embryos also express GnRH and GnRHR. Binding of GnRH to GnRHR causes the release of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from gonadotropic cells of the anterior pituitary. Mutations in GnRHR may cause hypogonadotropic hypogonadism and may be associated with anosmia.
Application: Functional assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, monitored measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Adelman et al. Two mammalian genes transcribed from opposite strands of the same DNA locus. Science 235:1514-1517, 1987.
Adelman et al. Isolation of the gene and hypothalamic cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat. Proc Nat Acad Sci 83:179-183, 1986.