MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT MRGX1/SNSR4 RECEPTOR

Product Information

Catalog Number:
C1256

Lot Number:
C1256-061505

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO dhfr- Gαqi5

Transfection:
Full-length human MRGX1 cDNA (GenBank Accession Number NM_147199)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: Alpha-MEM, 10% FBS, 100 µg/mL hygromycin, 800 µg/mL G418

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: MRGX1 (MAS-related GPR member X1) is also known as SNSR4 (sensory neuron-specific G-protein-coupled receptor 4). It can be potently activated by enkephalins including BAM22 and BAM(8-22). MRGX1 receptor is expressed solely in small diameter primary sensory neurons. This restricted expression pattern is of considerable therapeutic interest because small nociceptors transmit chronic pain messages.

Application: Functional assays

Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Chen and Ikeda (2004) Modulation of ion channels and synaptic transmission by a human sensory neuron-specific G-protein-coupled receptor, SNSR4/mrgX1, heterologously expressed in cultured rat neurons. J Neurosci 24:5044-5053.

Ahmad and Dray (2004) Novel G protein-coupled receptors as pain targets. Curr Opin Investig Drugs 5:67-70.

FOR RESEARCH USE ONLY.
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