MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MRGX1/SNSR4 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
CHO dhfr- Gαqi5
Full-length human MRGX1 cDNA (GenBank Accession Number NM_147199)
Liquid nitrogen upon receiving
Propagation Medium: Alpha-MEM, 10% FBS, 100 µg/mL hygromycin, 800 µg/mL G418
Stable after minimum of two months continuous growth
Background: MRGX1 (MAS-related GPR member X1) is also known as SNSR4 (sensory neuron-specific G-protein-coupled receptor 4). It can be potently activated by enkephalins including BAM22 and BAM(8-22). MRGX1 receptor is expressed solely in small diameter primary sensory neurons. This restricted expression pattern is of considerable therapeutic interest because small nociceptors transmit chronic pain messages.
Application: Functional assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Chen and Ikeda (2004) Modulation of ion channels and synaptic transmission by a human sensory neuron-specific G-protein-coupled receptor, SNSR4/mrgX1, heterologously expressed in cultured rat neurons. J Neurosci 24:5044-5053.
Ahmad and Dray (2004) Novel G protein-coupled receptors as pain targets. Curr Opin Investig Drugs 5:67-70.