MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT FPR2 RECEPTOR
Catalog Number: C1244a
Lot Number: C1244a-120120
Quantity: 1 vial (2 x 106) frozen cells
Freeze Medium: Cellbanker 2
Host cell: HEK293T
Transfection: Expression vector containing full-length human FPR2 cDNA (GenBank Accession Number NM_001462.3) with FLAG tag sequence at N-terminus
Recommended Storage: Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability: in progress
Background: The lipoxin A4 receptor ALX is also known as formyl peptide receptor-like 1 (FPRL1) or formyl peptide receptor 2 (FPR2). It shares 69% amino acid identity with FPR but displays low affinity for bacterial peptide N-formyl-methionyl-leucyl-phenylalanine (fMLF). ALX is highly expressed on neutrophils and monocytes and mediates cell chemotaxis. By interacting with a variety of exogenous and host-derived agonists, ALX may have important implications in the pathogenesis of human diseases such as HIV, Alzheimer’s disease (AD), amyloidosis and prion diseases.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with Multiscreen™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with Multiscreen™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Le et al. (2002) Formyl-peptide receptors revisited. Trends Immunol 23:541-548.
Migeotte et al. (2005) Identification and characterization of an endogenous chemotactic ligand specific for FPRL2. J Exp Med 201:83-93.