MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MTNR1B/MT2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human MTNR1B/MT2 (GenBank Accession Number NM_005959)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 200 µg/mL hygromycin, 1 µg/mL puromycin
Stable after minimum of two months continuous growth
Background: MT2 receptor, also known as MTNR1B (Melatonin receptor type 1B), is a high affinity receptor for melatonin. It is known to mediate the reproductive and circadian actions of melatonin and is considered as a novel therapeutic target for the development of subtype-selective analogs for the treatment of circadian sleep and mood-related disorders. MT2 receptor is also likely to mediate the interfering with the apoptotic pathway activated by heat shock in HL-60 cells.
Application: Functional assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Reppert et al. (1995) Molecular characterization of a second melatonin receptor expressed in human retina and brain: the Mel1b melatonin receptor. Proc Natl Acad Sci USA 92:8734-8738.
Cabrera et al. (2003) Melatonin prevents apoptosis and enhances HSP27 mRNA expression induced by heat shock in HL-60 cells: possible involvement of the MT2 receptor. J Pineal Res 35:231-238.
Dubocovich et al. (1998) Selective MT2 melatonin receptor antagonists block melatonin- mediated phase advances of circadian rhythms. FASEB J 12:1211-1220.