MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MT1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human MTNR1A cDNA (GenBank Accession Number NM_005958) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin
Stable in culture for minimum of two months
Background: MT1 (Melatonin receptor 1A, previously Mel 1A or ML 1A) is a receptor for melatonin. Melatonin is synthesized mainly in the pineal gland but is also made by other organs, such as the retina and the stomach. It is essential to the regulation of the mammalian circadian system and reproductive function. Melatonin receptor agonists may also be useful as a supplement to classical antidepressant treatments.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Audinot et al. (2003) New selective ligands of human cloned melatonin MT1 and MT2 receptors. Naunym Schmiedebergs Arch Pharmacol 367:553-561.
Browning et al. (2000) Pharmacological characterization of human recombinant melatonin mt1 and MT2 receptors. Br J Pharmacol 129:877-886.
Hirsch-Rodriguez et al. (2007) The pattern of melatonin receptor expression in the brain may influence antidepressant treatment. Med hypotheses 69:120-124.