MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT PAF RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human PTAFR cDNA (GenBank Accession Number NM_000952) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: The platelet-activating factor (PAF) receptor mediates a wide range of biological responses to PAF, a potent glycerophospholipid released from a variety of cell types such as stimulated basophils, platelets, polymorphonuclear neutrophils and macrophages. PAF is involved in a diverse array of biological activities related to inflammatory and immune responses as well as cardiovascular, respiratory and nervous system physiology. In humans, various diseases have been associated with PAF, such as allergic asthma, endotoxic shock, atherosclerosis and psoriasis.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Dupre et al. (2003) Trafficking, ubiquitination, and down-regulation of the human platelet-activating factor receptor. J Biol Chem 278:48228-48235.
Seyfried et al. (1992) The human platelet-activating factor
receptor gene (PTAFR) contains no introns and maps to chromosome 1. Genomics 13:832-834.
Van Biesen et al. (1996) G(o)-protein alpha-subunits activate mitogenactivated protein kinase via a novel protein kinase C-dependent mechanism. J Biol Chem271:1266-1269.