MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT FP RECEPTOR

Product Information

Catalog Number:
C1205

Lot Number:
C1205-051506

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human FP cDNA (GenBank Accession Number NM_000959) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable after minimum of 2 months continuous growth

Data Sheet

Background: Prostaglandin F (2-alpha) is involved in a number of physiologic processes. It serves as a potent luteolytic agent in many species, has been implicated as a modulator of intraocular pressure, smooth muscle contraction in the uterus and elsewhere. Its effects on cells are mediated through specific interaction with the prostaglandin F receptor (FP or PTGFR). Knockout mice lacking the FP receptor are unable to deliver normal fetuses at term due to a lack of response to oxytocin. The mice also failed to show the decline in serum progesterone expected to precede parturition.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Choe et al. (1998) The orphan seven-transmembrane receptor apj supports the entry of primary T-cell-line-tropic and dualtropic human immunodeficiency virus type 1. J Virol 72:6113-6118.

Youn et al. (2002) Role of the CC chemokine receptor 9/TECK interaction in apoptosis. Apoptosis 7:271-276.

FOR RESEARCH USE ONLY.
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