MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT FP RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human FP cDNA (GenBank Accession Number NM_000959) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of 2 months continuous growth
Background: Prostaglandin F (2-alpha) is involved in a number of physiologic processes. It serves as a potent luteolytic agent in many species, has been implicated as a modulator of intraocular pressure, smooth muscle contraction in the uterus and elsewhere. Its effects on cells are mediated through specific interaction with the prostaglandin F receptor (FP or PTGFR). Knockout mice lacking the FP receptor are unable to deliver normal fetuses at term due to a lack of response to oxytocin. The mice also failed to show the decline in serum progesterone expected to precede parturition.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
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