Product Information
Catalog Number:
C1200
Lot Number:
C1200-061006
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human DP cDNA (GenBank Accession Number NM_000953) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
Stable after minimum of 2 months continuous growth
Data Sheet
Background: The prostaglandin D2 receptor DP1 (DP or PTGDR) is a G protein- coupled receptor for the prostaglandin D2 (PGD2). PGD2 signals through DP2 (CRTH2) to induce TH2, eosinophil, and basophil chemotaxis in a Gαi-dependent manner. In contrast, signaling through DP1 is coupled to Gαs and does not induce chemotaxis.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Boie et al. (1995) Molecular cloning and characterization of the human prostanoid DP receptor. J Biol Chem 270:18910-18916.
Hirai et al. (2001) Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2. J Exp Med 193:255-261.