MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT DP1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human DP cDNA (GenBank Accession Number NM_000953) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of 2 months continuous growth
Background: The prostaglandin D2 receptor DP1 (DP or PTGDR) is a G protein- coupled receptor for the prostaglandin D2 (PGD2). PGD2 signals through DP2 (CRTH2) to induce TH2, eosinophil, and basophil chemotaxis in a Gαi-dependent manner. In contrast, signaling through DP1 is coupled to Gαs and does not induce chemotaxis.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation. Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with cAMP HiRange kit (Cisbio 62AM6PEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Boie et al. (1995) Molecular cloning and characterization of the human prostanoid DP receptor. J Biol Chem 270:18910-18916.
Hirai et al. (2001) Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2. J Exp Med 193:255-261.