MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GAL1 RECEPTOR
1 vial (2 x 106) frozen cells
Expression vector containing full-length human GALR1 cDNA (GenBank Accession Number NM_001480) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin
Stable after two months of continuous growth
Background: The diverse physiological effects of Galanin, a biologically active neuropeptide, are mediated through cell surface G protein-coupled receptors. To date, three galanin receptor subtypes, GALR1, GALR2 and GALR3, have been cloned. Galanin, widely distributed in the central and peripheral nervous systems and the endocrine systems, binds to galanin receptors to induce several regulatory functions in neuronal cells, including neuroregeneration, control of endocrine and exocrine secretions, and modulation of sensory and behavioral functions. Galanin agonists have been shown to have therapeutic application in treatment of chronic pain; galanin antagonists have therapeutic potential in treatment of Alzheimer’s disease, depression, and feeding disorders.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiScreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiScreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Branchek et al. (1998) Molecular biology and pharmacology of galanin receptors. Ann N Y Acad Sci 863: 94-107.
Wang et al. (1998) Differential intracellular signaling of the GalR1 and GalR2 galanin receptor subtypes. Biochemistry 37:6711-6717.