MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT P2Y12 RECEPTOR

Product Information

Catalog Number:
C1170-3a

Lot Number:
C1170-3a-020320

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2

Host cell:
1321N1

Transfection:
Expression vector containing full-length P2RY12 cDNA (GenBank Accession Number NM_022788) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: P2RY12 or P2Y12 is a G-protein coupled receptor for ADP and ATP. ADT released from platelets acts in a positive feedback mechanism on P2Y12 and P2Y1 on platelets and is critical for sustained aggregation and stabilization of thrombin. P2Y12 receptor plays a central role in platelet activation and is the target of of antithrombotic drugs such as ticlopidine and clopidogrel.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.

References:

Hardy et al. (2004) Reciprocal cross-talk between P2Y1 and P2Y12 receptors at the level of calcium signaling in human platelets. Blood 104:1745-1752.

Cattaneo et al. (2003) Molecular bases of defective signal transduction in the platelet P2Y12 receptor of a patient with congenital bleeding. Proc Natl Acad Sci USA 100: 1978-1983.

FOR RESEARCH USE ONLY.
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