MULTISCREEN™ STABLE CELL LINES

RAT RECOMBINANT P2Y12 RECEPTOR

Product Information

Catalog Number:
C1170-1

Lot Number:
C1170-1-102008

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO dhfr-

Transfection:
Full length rat R2RY12 cDNA (GenBank Accession Number N<_022800)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: α-MEM, 10% FBS, 100 μg/ml hygromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: P2RY12 or P2Y12 is a G-protein coupled receptor for ADP and ATP. ADT released from platelets acts in a positive feedback mechanism on P2Y12 and P2Y1 on platelets and is critical for sustained aggregation and stabilization of thrombin. P2Y12 receptor plays a central role in platelet activation and is the target of of antithrombotic drugs such as ticlopidine and clopidogrel.

Application: Functional assays

Figure Legend. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Hardy et al. (2004) Reciprocal cross-talk between P2Y1 and P2Y12 receptors at the level of calcium signaling in human platelets. Blood 104:1745-1752.

Cattaneo et al. (2003) Molecular bases of defective signal transduction in the platelet P2Y12 receptor of a patient with congenital bleeding. Proc Natl Acad Sci USA 100: 1978-1983.

FOR RESEARCH USE ONLY.
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