MULTISCREEN™ STABLE CELL LINES
RAT RECOMBINANT P2Y12 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full length rat R2RY12 cDNA (GenBank Accession Number N<_022800)
Liquid nitrogen upon receiving
Propagation Medium: α-MEM, 10% FBS, 100 μg/ml hygromycin
Stable after minimum of two months continuous growth
Background: P2RY12 or P2Y12 is a G-protein coupled receptor for ADP and ATP. ADT released from platelets acts in a positive feedback mechanism on P2Y12 and P2Y1 on platelets and is critical for sustained aggregation and stabilization of thrombin. P2Y12 receptor plays a central role in platelet activation and is the target of of antithrombotic drugs such as ticlopidine and clopidogrel.
Application: Functional assays
Figure Legend. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Hardy et al. (2004) Reciprocal cross-talk between P2Y1 and P2Y12 receptors at the level of calcium signaling in human platelets. Blood 104:1745-1752.
Cattaneo et al. (2003) Molecular bases of defective signal transduction in the platelet P2Y12 receptor of a patient with congenital bleeding. Proc Natl Acad Sci USA 100: 1978-1983.