MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT P2Y6 RECEPTOR

Product Information

Catalog Number:
C1164-3

Lot Number:
C1164-3-040611

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
1321N1

Transfection:
Expression vector containing full-length human P2Y6 cDNA (GenBank Accession Number NM_004154) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: The human P2Y6 receptor is a member of the G protein-coupled P2 receptor family that responds specifically to the extracellular nucleotide uridine diphosphate (UDP). The distribution of the P2Y6 receptor is widespread including heart, blood vessels and brain. P2Y6 receptors are significantly upregulated in T cells infiltrating regions of inflamed bowel and are involved in monocytic release of IL-8 in response to UDP or lipopolysaccharide (LPS). The immune effect of UDP/P2Y6 signaling on the production of proinflammatory cytokines has been shown to be selective and dependent on cell types.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Communi et al. (1996) Cloning, functional expression and tissue distribution of the human P2Y6 receptor. Biochem Biophys Res Commun 222:303-308.

Warny et al. (2001) P2Y(6) nucleotide receptor mediates monocyte interleukin-8 production in response to UDP or lipopolysaccharide. J Biol Chem 276:26051-26056.

FOR RESEARCH USE ONLY.
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