MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT P2Y4 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human P2Y4 cDNA (GenBank Accession Number NM_002565) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: P2Y4 is a 365-amino acid 7 transmembrane protein. It has been shown that, when expressed in a mammalian cell line, the receptor protein was activated specifically by UTP and UDP, but not by ATP and ADP. Activation of P2RY4 resulted in increased inositol phosphate formation and calcium mobilization. Adrian et al, anazlyed the espression of several purinergic receptors during differentiation in a promyelocytic leukemia cell line. Granulocytic differentiation was induced by dimethylsulfoxide, and a monocytic/macrophage phenotype was induced by phorbol esters. P2RY4 was highly expressed in uninduced promyelocytes, and its expression decreased slightly following both granulocytic and monocytic differentiation.
Application: Functional assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Adrian et al. (2000) Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells. Biochim Biophys Res Acta 1492: 127-138.
Nguyen et al. (1995) Cloning, expression and chromosomal localization of the human uridine nucleotide receptor gene. J Biol Chem 270:30845-30848.