MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT GPR85 RECEPTOR

Product Information

Catalog Number:
C1138

Lot Number:
C1138-020817

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
90%FBS, 10% DMSO

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GPR85 cDNA (GenBank Accession Number NM_018970.3) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: GPR85 (G protein-coupled receptor 85) is also known as SREB2 (Super Conserved Receptor Expressed in Brain 2) and belongs to the family of class A (rhodopsin-like) orphan G protein-coupled receptors. The receptor is reported to be highly expressed in the brain and testis. Research has linked GPR85 to influences of brain size, behavior, and vulnerability to schizophrenia.

Application: Functional assays

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Yanai et al. (2016) Identification and molecular docking studies for novel inverse agonists of SREB, super conserved receptor expressed in brain. Genes to Cells 21:717-727.

FOR RESEARCH USE ONLY.
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