MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT NPBW2 RECEPTOR

Product Information

Catalog Number:
C1133

Lot Number:
C1133-061505

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Full-length human NPBWR2 cDNA (GenBank Accession Number NM_005286)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 5 µg/mL blasticidine, 0.625 µg/mL puromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: Neuropeptide B/neuropeptide W receptor 2 (NPBW2 or GPR8) is one of the two receptors for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.

Application: Functional assays

Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Tanaka et al. (2003) Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8. Proc Natl Acad Sci USA 100:6251-6256.

Levine et al. (2005) Injection of neuropeptide W into paraventricular nucleus of hypothalamus increases food intake. Am J Physiol Regul Integr Comp Physiol288:R1727-R1732.

FOR RESEARCH USE ONLY.
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