MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPBW2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human NPBWR2 cDNA (GenBank Accession Number NM_005286)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 5 µg/mL blasticidine, 0.625 µg/mL puromycin
Stable after minimum of two months continuous growth
Background: Neuropeptide B/neuropeptide W receptor 2 (NPBW2 or GPR8) is one of the two receptors for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.
Application: Functional assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Tanaka et al. (2003) Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8. Proc Natl Acad Sci USA 100:6251-6256.
Levine et al. (2005) Injection of neuropeptide W into paraventricular nucleus of hypothalamus increases food intake. Am J Physiol Regul Integr Comp Physiol288:R1727-R1732.