MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT PK1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C- 6164)
Expression vector containing full-length human PK1 cDNA (GenBank Accession Number: NM_138964) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin
Stable in culture for minimum of two months
Background: Prokineticin receptor 1 (PK1 or GPR73) is a receptor for the cysteine-rich secreted peptides prokineticin 1 and 2. RT-PCR detected GPR73 expression in the brain, testis, small intestine, ovary, thyroid, pituitary and salivary gland.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose response of intracellular IP1 accumulation upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC).
Lin et al. (2002) Identification and molecular characterization of two closely related G protein-coupled receptors activated by prokineticins/endocrine gland vascular endothelial growth factor. J Biol Chem 277:19276-19280.