MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPBW1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human NPBWR1 cDNA (GenBank Accession Number NM_005285)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 200 µg/mL hygromycin, 0.625 µg/mL puromycin
Stable after minimum of 2 months continuous growth
Background: Neuropeptide B/neuropeptide W receptor 1 (NPBW1 or GPR7) is a receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Targeted disruption of NPBW1 in mice has confirmed that this receptor plays a role in maintaining long-term energy homeostasis. NPBW1 lacking male mice show moderately severe, late-onset obesity that is a result of both hyperphagia and decreased energy expenditure with reduced locomotor activity.
Application: Functional assays
Figure legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Levine et al. (2005) Injection of neuropeptide W into paraventricular nucleus of hypothalamus increases food intake. Am J Physiol Regul Integr Comp Physiol288:R1727-R1732.
Ishii et al. (2003) Targeted disruption of GPR7, the endogenous receptor for neuropeptides B and W, leads to metabolic defects and adult-onset obesity. Proc Natl Acad Sci USA 100: 0540-10545.