MULTISCREEN™ STABLE CELL LINES

RAT RECOMBINANT NPBW1 RECEPTOR

Product Information

Catalog Number:
C1124-1

Lot Number:
C1124-1-073005

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Full-length rat Npbwr1 cDNA (GenBank Accession Number NM_001014784)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 200 µg/mL hygromycin, 0.625 µg/mL puromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: Neuropeptide B/neuropeptide W receptor 1 (NPBW1 or GPR7) is a receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Targeted disruption of NPBW1 in mice has confirmed that this receptor plays a role in maintaining long-term energy homeostasis. NPBW1 lacking male mice show moderately severe, late-onset obesity that is a result of both hyperphagia and decreased energy expenditure with reduced locomotor activity.

Application: Functional assays

Figure Legend. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Levine et al. (2005) Injection of neuropeptide W into paraventricular nucleus of hypothalamus increases food intake. Am J Physiol Regul Integr Comp Physiol 288:R1727- R1732.

Ishii et al. (2003) Targeted disruption of GPR7, the endogenous receptor for neuropeptides B and W, leads to metabolic defects and adult-onset obesity. Proc Natl Acad Sci USA 100: 0540-10545.

FOR RESEARCH USE ONLY.
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