MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NMUR1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human NMUR1 cDNA (GenBank Accession Number NM_006056) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: NMUR1, or FM-3 or GPR66, is a receptor for the neuromedin U, a neuropeptide that has been implicated in physiological roles, including the regulation of feeding, anxiety, pain, blood flow, and smooth muscle contraction. The NMUR1 is abundantly expressed in peripheral tissues such as pancreas, testis and small intestine.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FlexStation. Figure 2. Dose-dependent accumulation of intracellular IP1 upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Howard et al. (2000) Identification of receptors for neuromedin U and its role in feeding. Nature 406:70-74.
Kojima et al. (2000) Purification and identification of neuromedin U as an endogenous ligand for an orphan receptor GPR66 (FM3). Biochem Biophys Res Commun 276:435-438.