MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT LPA3 (EDG7) RECEPTOR

Product Information

Catalog Number:
C1053-6

Lot Number:
C1053-6-081009

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
RH7777

Transfection:
Expression vector containing full-length human LPAR3 cDNA (GenBank Accession Number NM_012152) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 3 μg/mL puromycin

Stability:
Stable after minimum two months continuous growth

Data Sheet

Background: LPA and the structurally related lysophoslipid mediator sphingosine 1- phosphate (S1P) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1) are S1P receptors; others (e.g., EDG2) are LPA receptors. LPA3 receptor (EDG7) mediates responses preferentially to unsaturated LPA, whereas LPA2 receptor (EDG4) mediates responses to both saturated and unsaturated LPA.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Bandoh et al. (1999) Molecular cloning and characterization of a novel human G- protein-coupled receptor, EDG7, for lysophosphatidic acid. J. Biol. Chem. 274:27776- 27785.

Ye et al. (2005) LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing. Nature 435:104-108.

FOR RESEARCH USE ONLY.
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