MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT S1P3 RECEPTOR

Product Information

Catalog Number:
C1049-6

Lot Number:
C1049-6-080408

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
RH7777

Transfection:
Expression vector containing full-length human S1P3 cDNA (GenBank Accession Number NM_005226.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 3 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: S1P3 (sphingosine 1-phosphate receptor 3) or EDG3 (endothelial differentiation G-protein coupled receptor 3) is a receptor for the lysosphingolipid sphingosine 1-phosphate, a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. EDG receptors mediate pro- angiogenic activities, such as endothelial cell proliferation, chemotaxis, and vessel morphogenesis. S1P3, along with S1P1, is required for directing the organization of endothelial cells into capillary networks.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent accumulation of intracellular IP1 upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Takuwa et al. (2001) Subtype-specific, differential activities of the EDG family receptors for sphingosine-1-phosphate, a novel lysophospholipid mediator. Mol Cell Endocrinol 177:3-11.

FOR RESEARCH USE ONLY.
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