MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CRF2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CRHR2 cDNA (GenBank Accession Number NM_001883.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CRF2 is a member of the corticotrophin-releasing factor (CRF) receptor family. It is expressed in the brain, blood vessels and intestine. CRF plays an integral role in the coordinating endocrine and behavioral responses to stress as well as in the pathophysiology of several neurophsychiatric diseases such as depression, anxiety and addiction. CRF2 is involved in stress responses, cardiovascular function and gastric motility. Recent clinical data suggest that CRF- related agents may be promising in the treatment of various endocrine, psychiatric, neurologic and inflammatory diseases. CRF2 agonists may be useful in the treatment of upper-GI inflammatory diseases.
Application: Functional assays
Figure 1. Dose-dependent inhibition of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
De Souza (1995) Corticotropin-releasing factor receptors: physiology, pharmacology, biochemistry and role in central nervous system and immune disorders. Psychoneuroendocrinology 20:789-819.
Gravanis et al. (2005) The corticotropin-releasing factor. Curr Med Chem 12:1503- 1512.
Liaw et al. (1996) Cloning and characterization of the human corticotrophin-releasing factor-2 receptor complementary deoxyribonucleic acid. Endocrinology 137:72-77.