MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT PRRP RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human GPR10 cDNA (GenBank Accession Number BC095539)
Liquid nitrogen upon receiving
Propagation Medium: Alpha-MEM, 10% FBS, 500 µg/mL G418
Stable in culture for minimum of two months
Background: Prolactin-releasing peptide receptor PRRP (or GPR10) is detected in human brain, pituitaries, normal portions of adrenal glands and various tumor tissues. High level of expression is detected in pheochromocytomas. GPR10 was investigated regarding the effect of normal and high-fat diets on energy intake, body weight, and glucose homeostasis in wild-type and GPR10 knockout mice. The investigation provided direct evidence that GPR10 is involved in the regulation of energy balance.
Application: Functional assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Gu et al. (2004) The prolactin-releasing peptide receptor (GPR10) regulates body weight homeostasis in mice. J Mol Neurosci 22:93-103.
Takahashi et al. (2002) Expression of prolactin-releasing peptide and its receptor in the human adrenal glands and tumor tissues of adrenocortical tumors, pheochromocytomas and neuroblastomas. Peptides 23:1135-1140.