MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT UT RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human UTS2R cDNA (GenBank Accession Number NM_018949.1)
Liquid nitrogen upon receiving
Propagation Medium: DME/F12, 10% FBS, 10 µg/mL puromycin
Background: The urotensin 2 receptor (UT or GPR14) is high affinity receptor for urotensin-2 and urotensin-2B. In human, Urotensin II is the most potent vasoconstrictor known, possibly contributing to several human cardiovascular diseases. Both UT receptor and urotensin II are widely expressed in many other tissues, implicating urotensin II in the pathogenesis of a variety of disease processes ranging from hypertension to hepatic cirrhosis and UT antagonists for the possibility of a new range of therapeutic drugs.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Kemp et al. (2005) Urotensin II: a vascular mediator in health and disease. Curr Vasc Pharmacol 3:159-168.
Carotenuto et al. (2004) Urotensin-II receptor peptide agonists. Med Res Rev 24:577-588.