MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MCH2 (GPR145) RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human MCH- 2R/GPR145 cDNA (GenBank Accession Number NM_032503.1)
Liquid nitrogen upon receiving
Propagation Medium: Alpha-MEM, 10% FBS, 400 µg/mL G418
Stable in culture for minimum of two months
Background: Melanin-concentrating hormone receptor 2 (MCHR2, or GPR145) is the second MCH receptor identified in human. The primary function of both MCH receptors, MCHR1 and MCHR2, is to regulate skin color. In addition, they also regulate food intake, energy expenditure, behavior and emotion.
Application: Functional assays
Figure legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Mori et al. (2001) Cloning of a novel G protein-coupled receptor, SLT, a subtype of the melanin-concentrating hormone receptor. Biochem Biophys Res Commun 283:1013-1018.
Shi (2004) Beyond skin color: emerging roles of melanin-concentrating hormone in energy homeostasis and other physiological functions. Peptides 25:1605-1611.