MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT MCH1 RECEPTOR (GPR24)

Product Information

Catalog Number:
C1031-3

Lot Number:
C1031-3-073005

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Full-length human MCHR1 cDNA (GenBank Accession Number NM_005297)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 200 μg/mL hygromycin, 100 μg/mL zeocin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: MCHR1 or GPR24 is a receptor for melanin-concentrating hormone (MCH). MCH-1R is one of the two MCH receptors identified in human. The primary function of both MCH receptors, MCHR1 and MCHR2, is to regulate skin color. In addition, they also regulate food intake, energy expenditure, behavior and emotion. Blockade of MCHR1 could provide a viable approach for treatment of obesity and certain neurological disorders.

Application: Functional assays

Figure legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Mori et al. (2001) Cloning of a novel G protein-coupled receptor, SLT, a subtype of the melanin-concentrating hormone receptor. Biochem Biophys Res Commun 283:1013-1018.

Shi (2004) Beyond skin color: emerging roles of melanin-concentrating hormone in energy homeostasis and other physiological functions. Peptides 25:1605-1611.

FOR RESEARCH USE ONLY.
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