MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MCH1 RECEPTOR (GPR24)
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human MCHR1 cDNA (GenBank Accession Number NM_005297)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 200 μg/mL hygromycin, 100 μg/mL zeocin
Stable after minimum of two months continuous growth
Background: MCHR1 or GPR24 is a receptor for melanin-concentrating hormone (MCH). MCH-1R is one of the two MCH receptors identified in human. The primary function of both MCH receptors, MCHR1 and MCHR2, is to regulate skin color. In addition, they also regulate food intake, energy expenditure, behavior and emotion. Blockade of MCHR1 could provide a viable approach for treatment of obesity and certain neurological disorders.
Application: Functional assays
Figure legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Mori et al. (2001) Cloning of a novel G protein-coupled receptor, SLT, a subtype of the melanin-concentrating hormone receptor. Biochem Biophys Res Commun 283:1013-1018.
Shi (2004) Beyond skin color: emerging roles of melanin-concentrating hormone in energy homeostasis and other physiological functions. Peptides 25:1605-1611.