MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT H4 RECEPTOR

Product Information

Catalog Number:
C1030

Lot Number:
C1030-112009

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human HRH4 cDNA (GenBank accession number NM_021624.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: Histamine is one of the most studied biomolecules in medicine and is most notably known for its effects on smooth muscle contraction, vascular permeability and regulation of stomach acid. The histamine receptor H4 has been shown to have a role in chemotaxis and mediator release in a variety of immune cells, such as mast cells, eosinophils, dendritic cells, and T cells. The development of potent H4 receptor antagonists has great potential to open up the pathway for new therapeutic treatments in chronic inflammatory diseases, such as bronchial asthma, allergic gastrointestinal disease, and atopic dermatitis.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Daugherty (2004) Histamine H4 antagonism: a therapy for chronic allergy?. Br J Pharmacol 142:5-7.

Nguyen et al. (2001) Discovery of a novel member of the histamine receptor family. Mol Pharmacol 59:427-433.

FOR RESEARCH USE ONLY.
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