MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT H1 RECEPTOR

Product Information

Catalog Number:
C1027

Lot Number:
C1027-102007

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human HRH1 cDNA (GenBank Accession Number NM_000861) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: Histamine H1 receptor (H1 or HRH1) is a subclass of histamine receptors. It mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, and neurotransmission in the central nervous system. Antihistamines including H1 receptor antagonists are among the most frequently used pharmacologic agents because of a high incident rate of upper respiratory allergies. Clinical and laboratory evidence on histamine and its actions suggests that it has a pathophysiologic role in asthma. These findings have renewed interest in the potential therapeutic role of H1-receptor antagonists, such as, fexofenadine, which has been investigated as potential therapeutic targets for this disease.

Application: Functional assays

Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Cuss (1999) Beyond the histamine receptor: effect of antihistamines on mast cells. Clin Exp Allergy 29 Suppl 3:54-59

Gelfand (2002) Role of histamine in the pathophysiology of asthma: immunomodulatory and anti-inflammatory activities of H1-receptor antagonists. Am J Med 113 Suppl 9A:2S-7S.

FOR RESEARCH USE ONLY.
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