MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR10 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human CCR10 cDNA (GenBank accession Number NM_016602.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CCR10 (also known as GPR2) is selectively expressed by IgA- secreting cells including circulating IgA+ plasmablasts, as well as almost all IgA+ plasma cells in the salivary gland, small intestine, large intestine, appendix and tonsils. It is a receptor for CCL27/CTACK and CCL28/MEC. Expression of CCL27 in skin may play an important role in facilitating memory T cells that express CCR10 to enter into cutaneous sites during inflammation and in determining the metastatic destination of malignant melanoma.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation. Figure 2. Dose-dependent inhibition of forskolin- stimulated intracellular cAMP level upon treatment with ligand, measured with cAMP HiRange kit (Cisbio 62AM6PEC). Figure 3. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Muller et al. (2001) Involvement of chemokine receptors in breast cancer metastasis. Nature 410:50-56.
Kunkel et al. (2003) CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells. J Clin Invest 111:1001-1010.
Hudak et al. (2002) Immune surveillance and effector functions of CCR10(+) skin homing T cells. J Immunol 169:1189-1196.