MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR7 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR7 cDNA (GenBank Accession Number: NM_001838) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CCR7 (C-C chemokine receptor type 7 is a receptor for the chemokine MIP-3β. CCR7 has been identified as a key regulator of homeostatic B and T cell trafficking to secondary lymphoid organs. CCR7 is also an essential mediator for entry of both dermal and epidermal dendritic cells into the lymphatic vessels within the dermis. CCR7 plays an important role in tumor cell migration and lymph node metastasis. Expression of CCR7 is related to the development of lymph node metastasis in nonsmall cell lung cancers.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Takanami (2003) Overexpression of CCR7 mRNA in nonsmall cell lung cancer: correlation with lymph node metastasis. Int J Cancer 105:186-189.
Ohl et al. (2004) CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions. Immunity 21:279-288.