MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR6 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR6 cDNA (GenBank Accession Number NM_004367) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CCR6 is a receptor for macrophage inflammatory protein (MIP)-3α (CCL20). It is expressed in immature dendritic cells as well as in B lymphocytes and memory T cells. In gene-targeted mice lacking CCR6, myeloid dendritic cells are either absent or underrepresented in the subepithelial dome compared with wild-type mice. CCR6 and its ligands contribute to the normal homeostatic regulation of extrathymic T cell development in the intestine.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Baba et al. (1997) Identification of CCR6, the specific receptor for a novel lymphocytedirected CC chemokine LARC. J Biol Chem 272:14893-14898.
Cook et al. (2000) CCR6 mediates dendritic cell localization, lymphocyte homeostasis, and immune responses in mucosal tissue. Immunity 12:495-503.
Lugering et al. (2003) Lymphoid precursors in intestinal cryptopatches express CCR6 and undergo dysregulated development in the absence of CCR6. J Immunol 171:2208-2215.